商品编号


HTRH-100



品牌


MCLab



公司


MCLAB.Inc



公司分类


RNases



Concentration

5 U/?l


Size

1,250 units

商品信息


Thermo
stable RNase H?
degrades the RNA strand in RNA-DNA hybrids
has, it has an optimal activity above 65°C and can be used up to 95°C.




Description:

Thermo
stable RNase H has an optimal activity above 65°C and can be used up to 95°C. The enzyme degrades RNA in a DNA:RNA hybrid, maximizing sensitivity and selectivity without affecting DNA or unhybridized RNA.
Application:
- High hybridization stringency
- Specific hydrolysis of RNA in a DNA:RNA hybrid
- Diagnostic assay of DNA sequences by isothermal probe amplification
- Mapping of mRNA structures
Source:
A recombinant protein purified from
E. coli
, cloned the gene encoding the
Thermus
Thermo
philus
RNase H.
Unit Definition:
One unit of the enzyme results in the acid-solubilization of 1 nmol of polyadenylic acid in the presence of an equimolar concentration of polythymidylic acid in 20 minutes at 45°C in 50 mM Tris-HCl(pH 7.5), 100 mM NaCl, and 10 mM MgCl
2
. Note: The unit assay is performed at 45°C because this is optimal for the Tm of poly(dT):poly(A). The optimal temperature for many applications may be considerably higher.
10X RNase H Reaction Buffer
500 mM Tris-HCl(pH 7.5)
1 M NaCl
100 mM MgCl
2
pH 7.6 @ 25°C
Storage Buffer:
Supplied in 50% Glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1.0 mM DTT, 0.1 mM EDTA, and 0.1% Triton X-100.
Recommended Storage Condition:
-20°C