商品编号


RNIE-100



品牌


MCLab



公司


MCLAB.Inc



公司分类


RNases



Concentration

24,000 U/ml


Size

6,000 units

商品信息

RNase I catalyzes the hydrolysis of single-stranded RNA to nucleoside 3’-monophosphates via 2’, 3’ cyclic monophosphate intermediates.




Description:
RNase I catalyzes the hydrolysis of single-stranded RNA to nucleoside 3'-monophosphates via 2', 3' cyclic monophosphate intermediates. Note: The enzyme is inactivated by heating at 70°C for 15 minutes, eliminating phenol extractions to remove the enzyme.
Application:
- Degradation of single-stranded RNA to mono-, di- and trinucleotide
- Used in ribonuclease protection assays
Source:
An
E. coli
strain containing a genetic fusion of the RNase I gene (rna) from
E. coli
and the gene coding for maltose-binding protein (MBP).
Unit Definition:
One unit of enzyme required to catalyze the degradation of 100 ng of
E. coli
ribosomal RNA per second into acid-soluble nucleotides at 37°C.
Storage Buffer:
Supplied in 50% Glycerol, 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.01 mM EDTA.
Recommended Storage Condition:
-20°C