商品编号


TDL-100



品牌


MCLab



公司


MCLAB.Inc



公司分类


DNA/RNA Ligases



Concentration

40,000 U/ml


Size

10,000 units

商品信息


Taq
DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini, using NAD+ as a cofactor.





Description:
Taq
DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini, using NAD
+
as a cofactor.
Figure:
Figure, Comparing to another vendor's product, the activity of
MCLAB
's
Taq
DNA ligase was tested with
Bst
EII-digested Lamda DNA under 45°C for 15 minutes.
Application:
- Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction
- Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification
Source:
A recombinant
E. coli
strain carrying the cloned
Taq
DNA Ligase gene.
Supplied in:
10 mM Tris-HCl
50 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
0.1% TWEEN 20
50% Glycerol
pH 7.4 @ 25°C
Supplied with:
10x
Taq
DNA Ligase Buffer
10x Taq DNA Ligase Buffer:
200 mM Tris-HCl
250 mM Potassium Acetate
100 mM Magnesium Acetate
10 mM NAD 1
100 mM DTT
0.1% Triton? X-100
pH 7.6 @ 25°C
?


Unit Definition:


One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1 ?g of BstEII-digested λ DNA in a total reaction volume of 50 ?l in 15 minutes at 45°C.


Specific Activity:
?400
?
Unit/
?
g


Recommended Storage Condition:
-20°C