商品编号


2720



品牌


Chimerx



公司


Chimerx,Inc.



公司分类


PCR Products




200 u

商品信息

Details


Description


Perpetual OptiTaq DNA Polymerase is a modified and balanced blend containing top quality Thermus aquaticus DNA Polymerase, Pyrococcus furiosus DNA Polymerase, anti-Taq DNA Polymerase antibodies, revers
IBL
e inhibitors and enhancers.

Ultrapure, recombinant proteins are used to prepare Perpetual OptiTaq DNA Polymerase.

Our carefully selected anti-Taq antibodies have high thermal st
ABI
lity, providing protection against non-specific primer extension from room temperature to 70°C.

The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 92-95°C for two minutes.

Formation of inactive complexes between Taq DNA Polymerase and an anti-Taq antibody forms a basis for automatic "hot start" PCR, which allows for the assembly of PCR reactions at room temperature.

High st
ABI
lity of the complexes allows for the enormous increase of PCR specificity, sensitivity and yield in comparison to the conventional PCR assembly method.

Automatic "hot start" PCR is a convenient method when assembling multiple PCR reactions, saving time and reagents.

Clean and safe laboratory practice assured, due to removed necessity to open hot tubes.

The blend catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction in the presence of magnesium ions and exhibits the 3'→5' proofre
ADI
ng activity, resulting in considerably higher PCR fidelity than poss
IBL
e with unmodified Taq DNA polymerase (1).

Enables increased amplification product yield in comparison with Taq DNA polymerase over wide range of PCR products.

Maintains the 5'→3? exonuclease activity.

Adds extra A at the 3' ends.

Suitable for multiplex PCR due to increased specificity, wider tolerance for Mg
2+
, salts concentration, and pH (2,3).

Improves PCR results with critical templates, such as containing GC-rich regions, palindromes or multiple repeats.

Perpetual OptiTaq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 20 kb.


Storage Buffer

20 mM Tris-HCL (pH 8.0 at 22°C)
100 mM KCI
0.5% Igepal CA-630
0.1 mM EDTA
1 mM dithiothreitol
50% glycerol

10X Reaction Buffers

10X Pol Buffer A (optimization buffer without MgCl
2
)
The buffer allows to optimize MgCl
2
concentration.

10X Pol Buffer B (general application without MgCl
2
)
The buffer contains 15 mM MgCl
2
and is optimized for use with 0.2 mM of each dNTP.

10X Pol Buffer C (colored)
10X Pol Buffer B enriched with two gel tracking dyes and a gel lo
ADI
ng reagent. The buffer enables direct lo
ADI
ng of PCR products onto an agarose gel.

Quality Control

All preparations are assayed for contaminating endonuclease, 3'-exonuclease, and nonspecific singe- and double-stranded DNase activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.

References

(1) Cline, J., Braham, J. and Hogrefe, H. (1996) Nucleic Acids Res. 24, 3545.
(2) Chien, A., Edgar, D.B. and Trela, J.M. (1976) J. Bacteriol. 127, 1550.
(3) Kaledin, A.S., Silusarenko, A.G. and Gorodetskii, S.I. (1980) Biokhimiya 45, 644.