商品编号


R0156S-10,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

20,000 units/ml


Size

10,000 units

商品信息

Description:
SacI has a High Fidelity version SacI-HF? (
NEB
#R3156).
High Fidelity (HF?) Restriction Enzymes have 100% activity in CutSmart? Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.HF enzymes also exhibit dramatically reduced star activity.HF enzymes are all Time-Saver? qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA.Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Product Source
An
E. coli
strain that carries the SacI gene from
Streptomyces achromogenes
(
ATCC
12767).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
NEB
uffer? 1.1
-20
10X
Notes:
SacI is inhibited by salt concentrations > 10 mM. Mini-prep DNA containing residual salt is resistant to cleavage. A 70% alcohol wash or dialysis can be used to remove the salt.This enzyme has shown to have lower activity on some supercoiledplasmids, with more than 1 unit required to digest 1 μg plasmid DNA. Forcomplete digestion of 1 μg of plasmid DNA please follow our recommendeddigestion protocol.