商品编号


M0491S-500 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


PCR, qPCR & Amplification Technologies




500 units


Concentration

2,000 units/ml



商品信息

Description:
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Q5? High-Fidelity DNA Polymeraseis a high-fidelity,
Thermo
stable DNA polymerasewith 3?→ 5? exonuclease activity, fused to aprocessivity-enhancing Sso7d domain to supportrobust DNA amplification. With an error rate ~280-fold lower than that of
Taq
DNA Polymerase, Q5 High-FidelityDNA Polymerase is ideal for cloning and canbe used for long or difficult amplicons. Q5High-Fidelity DNA Polymerase is supplied withan optimized buffer system that allows robustamplification regardless of GC content. The 5XQ5 Reaction Buffer contains 2 mM Mg
++
at final(1X) reaction concentrations and is recommendedfor most routine applications. For GC-rich targets(≥ 65% GC), amplification can be improved bythe addition of the 5X Q5 High GC Enhancer. Q5High-Fidelity DNA Polymerase is unlike typical,lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the
NEB
Tm Calculatoris highly recommended.
Amplification of a variety of human genomic amplicons from low to high GC content using Q5 High-Fidelity DNA Polymerase. All reactions were conducted using 30 cycles of amplification and visualized by microfluidic LabChip? analysis.
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Product Source
An
E. coli
strain that carries the Q5 High-Fidelity DNA Polymerase gene.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
Q5? High GC Enhancer
5X
Q5? Reaction Buffer Pack
-20
5X