商品编号


R0123S-3,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

20,000 units/ml


Size

3,000 units

商品信息

Description:
SfiI crystals (Lydia Dorner and Ira Schildkraut,
New England Biolabs
)
Product Source
An?
E. coli
strain that carries the SfiI gene from
Streptomyces fimbriatus
(
ATCC
15051).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
SfiI requires two copies of its recognition sequence for cleavage to occur. The two sites can be on either the same or different DNA molecules. Wertzell L.M. et al., (1995) J. Mol. Biol. 248:581-595. For more information and recommendations on working with enzymesrequiring multi-sites, please visit Restriction Enzyme Cleavage:‘single-site’ enzymes and ‘multi-site’ enzymesFor enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch? PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.