商品编号


R0561S-1,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

10,000 units/ml


Size

1,000 units

商品信息

Description:
Product Source
An
?E. coli
?strain that carries the cloned SfcI gene from?
Streptococcus faecium
?(A. Meloni)
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
SfcI concentrations of < 1 unit/?g DNA in a reaction are not recommended.?SfcI is active for one hour in a reaction at 37°C, after one hour no further digestion takes place. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.The st
ABI
lity of SfcI is greatly enhanced by incubations at 25°C.Although the enzyme is only 25% as active at this temperature, werecommend an incubation temperature of 25°C for 4 - 16 hours whendigesting supercoiled plasmids. At 25°C, 0.1 unit will digest 1?g ofsubstrate DNA in 16 hours.Star activity may result from a glycerol concentration of >5%