商品编号


R0551S-1,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

10,000 units/ml


Size

1,000 units

商品信息

Description:
Product Source
An?
E. coli
?strain that carries the cloned AciI gene from?
Arthrobacter citreus
?(C. Polisson)
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
AciI has a non-palindromic recognition site. As a result, when DNA is cut with AciI and then ligated, only 50% of these ligated sites regenerate an AciI site.This enzyme does not benefit from incubations over 1 hour. Visit "Restriction Endonucleases-Survival in a Reaction" for more information.For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch? PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.