商品编号


GK80040



品牌


ProZyme



公司


ProZyme, Inc



公司分类


Glyko Enzymes



Size

1 U

商品信息

Description

Releases
?α(2,3)-, α(2,6)-, α(2,8)-, and α(2,9)-linked N-acetylneuraminic acid from?oligosaccharides and glycoproteins.?Also capable of releasing N-glycolylneuraminic acid.

Source:
Recombinant gene from
Arthrobacter ureafaciens
, expressed in
E. coli
.

Specificity:
The enzyme releases α(2,3)-, α(2,6)-, α(2,8)-, and α(2,9)-linked N-acetylneuraminic acid from complex carbohydrates. The initial rate of hydrolysis of α(2,6) linkages is reported to be approximately twice that of α(2,3)-linked sialic acid however, in practice, this kinetic selectivity is of little consequence during extended incubations. Effective digestion of glycolipid substrates is facilitated by addition of a detergent, such as sodium taurodeoxycholate to the incubation.

Sialidase A is capable of releasing N-glycolylneuraminic acid (Neu5Gc, NGNA) in addition to N-acetylneuraminic acid (Neu5Ac, NANA) [1], although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac.

The enzyme can also be used to remove sialic acid from gangliosides [3], glycosphingolipds (
ceramide & oligosaccharide) with sialic acid.

May be used for exoglycosidase sequencing in conjunction with Sialidase S (
GK80021
), which is specific for α(2,3)-linked sialic acids.

Sialidases are also known as neuraminidases.

Ships with:

WS0049 5x Reaction Buffer B [250 mM sodium phosphate pH 6.0]

Reaction Buffer:
5X concentrated buffer which when diluted gives 50 mM sodium phosphate pH 6.0.

Formulation:

20 mM Tris HCl pH 7.5, containing 25 mM NaCl.

pH optimum:
6.0

Unit Definition:

One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5.

Size:
1 U (200 ?l)

Concentration:
?≥ 5 U/ml

Product Code:
GK80040



Uchida Y, Tsukada Y, Sugimori T. ?
Enzymatic properties of neuraminidases from Arthrobacter ureafaciens.
?
J Biochem.
1979 Nov;86(5):1573-85.


Schauer R. ?
Chemistry, metabolism, and
BIOLOG
ical functions of sialic acids.
?
Adv Carbohydr Chem Biochem.
1982;40:131-234.

Larsson EA, Olsson U, Whitmore CD, Martins R, Tettamanti G, Schnaar RL, Dovichi NJ, Palcic MM, Hindsgaul O. ?
Synthesis of reference standards to enable single cell metabolomic studies of tetramethylrhodamine-labeled ganglioside GM1.
?
Carbohydr Res.
2007 Feb 26;342(3-4):482-9