商品编号


GKE-5011B



品牌


ProZyme



公司


ProZyme, Inc



公司分类


Glyko Enzymes



Size

10 mU

商品信息

Description

Cleaves N-linked glycans from glycopeptides, including glycans with
α(1,3)-linked core fucose as seen in insects and plants.
?Peptide N-Glycosidase A, Glycopeptidase from almonds, Almond glycoamidase, Peptide-N(4)-(N-acetyl-β glucosaminyl) asparagine amidase.

Source:
Almond.

Specificity:

PNGase A from almonds cleaves N-Glycan chains linked to Asparagine from glycopeptides. The enzyme hydrolyzes an N(4)-(acetyl-β D-glucosaminyl) asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-β D-glucosaminylamine and a peptide containing an aspartate residue.

The enzyme cleaves N-glycans with or without α(1,3)-linked core fucose residues present in insect and plant glycoproteins. This differs from PNGase F, which cannot remove N-glycans with
α(1,3)-linked core fucose.
?PNGase A requires glycopeptides as substrates. Glycopeptides can be obtained by digesting the glycoprotein with trypsin [1,2] or pepsin. Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino acid residues in the substrate.

Ships with:
?WS0273?Reaction buffer: 50 mM citrate-phosphate buffer, pH 5.0, 0.02% sodium azide.

Formulation:
Lyophilized powder containing citrate-phosphate buffer, pH 5.0.

pH Range:
Optimum: pH 5.0 Range: pH 4.0 - 6.0

Unit Definition:
One unit is defined as the amount of enzyme required to hydrolyze 1 μmol ovalbumin glycopeptide per min at pH 5.0 and 37°C.

Size:
10 mU

?Product Code:?
GKE-5011B

1.?Küster B, Wheeler SF, Hunter AP, Dwek RA, Harvey DJ.?Sequencing of N-linked oligosaccharides directly from protein gels: in-gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography.?
Anal Biochem.
1997 Jul 15;250(1):82-101.

2.?Kolarich D, Altmann F.?N-Glycan analysis by matrix-assisted laser desorption/ionization mass spectrometry of electrophoretically separated nonmammalian proteins: application to peanut allergen Ara h 1 and olive pollen allergen Ole e 1.?
Anal Biochem.
2000 Oct 1;285(1):64-75.