商品编号





品牌


Ursabioscience



公司


Ursa BioScience



公司分类


Non - conjugatable


商品信息

Description
Viscous Blue 420? is a water soluble probe which can re
ADI
ly be excited in the range 250-400 nm, with λ
max
Abs ≈ 330 nm in Methanol and a blue emission band centered at ≈ 420 nm (glycerol). At 20 C the lifetime is bi-exponential (in glycerol), 0.899 ns (56%), and 1.70 ns (44%), with a mean lifetime of 1.13 ns. At 50 C the lifetimes are much shorter, 0.492 ns (82.7%) and 1.16 ns (17.3%), with a mean lifetime of 0.547 ns.
Viscous Blue 420? has a viscosity dependent emission centered at 420 nm, the intensity of which is dependent on viscosity, over a broad range of viscosity. As solution viscosity increases (solution temperature decreases), the fluorescence increases.
Viscous Blue 420? is sold dry and is simply reconstituted by adding water/MeOH/EtOH and/or buffer. One vial of our Viscosity Probe will typically allow for 10 measurements to be undertaken; 50 ?L of water or MeOH and/or buffer is typically added to each dry vial. Subsequently, ten 5 ?L aliquots diluted to 3 cm3, typically give an optical density > 0.1 at 330 nm (in MeOH).
For optimum performance, probe vials should be stored at -2 to -8 C when not being used. Probe solutions should be used after dilution.
An Ursa BioScience short video describes our reconstitution procedure.