商品编号


GKE-5003



品牌


ProZyme



公司


ProZyme, Inc



公司分类


Glyko Enzymes



Size

100 mU

商品信息

Description

Native PNGase F, peptide-
N
-glycosidase F, peptide-
N
4
-(
N-
acetyl-β-glucosaminyl)asparagine amidase.?Releases intact N-glycans by cleaving?between the innermost GlcNAc and Asn.? Also available as a recombinant product, see
GKE-5006A
.

Source:
Native, purified from?
Elizabethkingia
meningosepticum
. The source organism was previously known as?
Chryseobacterium [Flavobacterium] meningosepticum
[1].?

Specificity:
PNGase F
?releases intact N-linked oligosaccharides from glycoproteins and glycopeptides. The enzyme will not cleave oligosaccharides from a single asparagine residue and cleavage does not occur if there is core α1-3 linked fucose as commonly encountered in plant glycoproteins Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage.





?





Figure 1:

Cleavage specificity for GKE-5003 N-Glycanase (PNGase F).
R
1
: N & C substitution other than H
R
2
:?may be H or the rest of an oligosaccharide
R
3
:?may be H or?
α(1-6)-linked fucose

Applications:



release of intact N-linked glycans from glycopeptides and glycoproteins

structure-function studies of N-glycosylated glycoproteins

preparation of deglycosylated proteins for molecular weight estimation or crystallography studies



Ships with:
?



1 ea WS0067 5x Reaction?Buffer

1 ea WS0012 Denaturation Solution

1 ea WS0013 Detergent Solution



Formulation:
52 mM Tris-HCl, 36 mM NaCl, 9 mM sodium acetate, 5 mM EDTA, 0.02% sodium azide (pH 7.5), glycerol-free formulation

pH optimum:
7.5

Unit Definition:
One unit of N-Glycanase is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at pH 7.5 and 37?C.

Size:
100 mU (50 ?l)

Concentration:
?≥ 2 U/ml

Product Code:
GKE-5003

1. Kim KK, Kim MK, Lim JH, Park HY, Lee ST.?Transfer of
Chryseobacterium meningosepticum
and
Chryseobacterium miricola
to
Elizabethkingia
gen. nov. as
Elizabethkingia meningoseptica
comb. nov. and
Elizabethkingia miricola
comb. nov.?
Int J Syst Evol Microbiol.
2005 May;55(Pt 3):1287-93.