商品编号


M3030



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Ubiquitin / UBL Derivatives



Size

50 ?g


商品信息

This products is a fluorescence polarization (FP) assay reagent, which is based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitine via a native isopeptide bond with the lysine side-chain. Typical substrate concentrations range from 10?100 nM. DUB concentrations can range from 0.01-10 nM but depend on specific assay conditions and method of detection. FP assays can be performed with any fluorescence polarization reader with a 530 nm excitation filter and two 580 nm emission filters.

Additional Information






Product Name:

TAMRA-Lys(Ub)-Gly



Also Known As:

N/A



Catalog No.:

M3030



Size:

50 ?g



Molecular Weight:

9163 Da by MS



Species:

Human



Source:

Synthetic



Stock:

Product in lyophilized powder form.



Concentration:

N/A



Quality Assurance:

≥95% by RP-HPLC and SDS-PAGE



Storage:

Powder at ?20°C; solution at ?80°C. Avoid multiple freeze/thaw cycles.



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

N/A



Image(s):

(Click image to enlarge)











Shipping Method:

Room temperature shipping



References:

1. Tirat, A.
et al
., (2005) Anal. Biochem. 343, 244.
2. Huang
et al
., (2009) Methods in Molecular
BIOLOG
y 565, 127. 3. Levine
et al
., (1997) Anal. Biochem. 247, 83.
4. Geurink and El Oualid
et al
., (2012) ChemBiochem 13, 293.




Details

This products is a fluorescence polarization (FP) assay reagent, which is based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitine via a native isopeptide bond with the lysine side-chain. Typical substrate concentrations range from 10?100 nM. DUB concentrations can range from 0.01-10 nM but depend on specific assay conditions and method of detection. FP assays can be performed with any fluorescence polarization reader with a 530 nm excitation filter and two 580 nm emission filters.

Sample Preparation:
Dissolve the powder in DMSO to a final concentration of 1mM (50 μg/5.5 μl) and add this DMSO stock slowly to the required buffer (please note the order of addition) to a final concentration of 0.1 - 1 μM. The concentration of 5-TAMRA-Lys(Ub)-Gly-OH can be verified by comparing the fluorescence intensity with that of a known concentration of TAMRA.
Images:
(Click image to enlarge)