商品编号


P1001-200



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Deubiquitinating Enzymes



Size

200 ?g


商品信息

Tobacco Etch Virus (TEV) protease is a cysteine protease that is commonly used to remove fusion tags, including His, GST and MBP, at the N or C termini of recombinant proteins. TEV protease specifically recognizes a seven amino acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser (ENLYFQG/S) and cleaves between Gln and Gly/Ser under a wide range of conditions.

Recombinant 6xHis-TEV was expressed in E. Coli and purified to near homogeneity. A Ser219Asn (S219N) substitution was introduced that has no effect on TEV protease activity, but inhibits auto-cleavage and thus, st
ABI
lizing TEV protease during incubation and long-term storage.

We recommend using 3% supplied TEV protease (w/w) to preform reactions at 4 0C for 12-16 h or at 30 0C for 1-4 h. Depending on specific substrates, TEV protease concentration, incubation time, and reaction temperature should be optimized. 6xHis-TEV can be removed by Ni-NTA resin after incubation.

TEV protease maintains high activity under a wide range of temperatures (4 0C - 30 0C), pH (6.0 - 8.5) and buffers, and in the presence of commonly used protease inhibitors.


Additional Information






Product Name:

6xHis-TEV Protease



Also Known As:

TEV protease, NIa, Tobacco etch virus protease



Catalog No.:

P1001-200



Size:

200 ?g



Molecular Weight:

27 kDa



Species:

Tabacco Etch Virus



Source:

Bacterial recombinant



Stock:

100 mM Tris, pH 8.5 at 4 0C, 500 mM NaCl, 5mM DTT, 0.5 mM EDTA and 50% Glycerol



Concentration:

See tube label



Quality Assurance:

> 90% purity based on SDS-PAGE.



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

N/A



Image(s):




Shipping Method:

Dry ice shipping



References:

1. Lucast LJ.,
et al
. (2001) Biotechniques 30, 544.




Details

Tobacco Etch Virus (TEV) protease is a cysteine protease that is commonly used to remove fusion tags, including His, GST and MBP, at the N or C termini of recombinant proteins. TEV protease specifically recognizes a seven amino acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser (ENLYFQG/S) and cleaves between Gln and Gly/Ser under a wide range of conditions. Recombinant 6xHis-TEV was expressed in E. Coli and purified to near homogeneity. A Ser219Asn (S219N) substitution was introduced that has no effect on TEV protease activity, but inhibits auto-cleavage and thus, st
ABI
lizing TEV protease during incubation and long-term storage. We recommend using 3% supplied TEV protease (w/w) to preform reactions at 4 0C for 12-16 h or at 30 0C for 1-4 h. Depending on specific substrates, TEV protease concentration, incubation time, and reaction temperature should be optimized. 6xHis-TEV can be removed by Ni-NTA resin after incubation. TEV protease maintains high activity under a wide range of temperatures (4 0C - 30 0C), pH (6.0 - 8.5) and buffers, and in the presence of commonly used protease inhibitors.