商品编号


J6110



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Deubiquitinating Enzymes



Size

1 Kit

商品信息

This kit is designed to determine DUB inhibitor specificity and potency. You can select 10 DUBs of your choice from our DUB list. Each DUB is sufficient for 20 X 50ul reactions using a working concentration of 10nM. The supplied Ub-rhodamine 110 is sufficient for 550 X 50ul reactions using a working concentration of 100nM. The supplied iodoacetamide can be used as an inhibitor of cysteine DUBs using a working concentration of 10mM.

Additional Information






Product Name:

Deubiquitinating Enzyme Inhibitor Profiling Kit I



Also Known As:

N/A



Catalog No.:

J6110



Size:

1 Kit



Molecular Weight:

N/A



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

90% - 95% based on SDS-PAGE



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF datasheet



NCBI RefSeq:

N/A



Image(s):

N/A



Shipping Method:

Dry ice shipping



References:

N/A




Details

* When place an order, please select any ten (10) DUBs from the list on the right side of this page.

Description
This kit is designed to determine DUB inhibitor specificity and potency. You can select 10 DUBs of your choice from our DUB list. Each DUB is sufficient for 20 X 50ul reactions using a working concentration of 10nM. The supplied Ub-rhodamine 110 is sufficient for 550 X 50ul reactions using a working concentration of 100nM. The supplied iodoacetamide can be used as an inhibitor of cysteine DUBs using a working concentration of 10mM.

Components
? 10X DUBs (pick 10 DUBs from the list, 200nM)..................50 ?l each ? Ub-rhodamine 110 (1mg/ml)............................................................25 ?l
? Iodoacetamine (IAA).........................................................................25 mg ? 5X Deubiquitination Buffer.........................................................2X1.25ml

DUBs List
1. 6xHis-Uch37
2. Adrm1/Uch37 3. 6xHis-
USP
14
4. UCHL1 5. GST-UCHL1
6. UCHL3 7. GST-UCHL3
8. GST-Ataxin3(Q22) 9. OTUB1
10. GST-OTUB1 11. OTUB2
12. GST-OTUB2 13. GST-A20(1-365)
14. OTUD2 15. OTUD6B
16. 6xHis-OTULIN 17. GST-
USP
4
18.
USP
7 19. GST-
USP
7
20. GST-
USP
9X (catalytic domain) 21. GST-
USP
11
22. GST-
USP
15 23. GST-
USP
21(195-565)
24. GST-
USP
30(57-517)