商品编号


F1400-5



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Inhibitors



Size

5 mg


商品信息

Epoxomicin, originally isolated from
Actinomyces
sp., is a cell-permeable, potent, selective and irrevers
IBL
e proteasome inhibitor. It inhibits chymotrypsin-like, trypsin-like and caspase-like activities of the proteasome.

Additional Information






Product Name:

Epoxomicin



Also Known As:

CAS# 134381-21-8



Catalog No.:

F1400-5



Size:

5 mg



Molecular Weight:

554.72Da



Species:

N/A



Source:

Synthetic



Stock:

Powder



Concentration:

N/A



Quality Assurance:

>98% by HPLC and NMR



Storage:

Elig
IBL
e for room temperature shipping. Store at -80°C upon receiving; avoid multiple freeze-thaw cycles after dissolving in DMSO.



PDF Data Sheet:

Download PDF datasheet



NCBI RefSeq:

N/A



Image(s):

No



Shipping Method:

Room temperature shipping



References:

1. M. Hanada,
et al
. (1992) J. Antibiot., 45, 1746
2. L. Meng,
et al
. (1999) PNAS, 96, 10403
3. K.B. Kim,
et al
. Bioorg. (1999) Med. Chem. Lett., 9, 3335




Details

Epoxomicin, originally isolated from
Actinomyces
sp., is a cell-permeable, potent, selective and irrevers
IBL
e proteasome inhibitor. It inhibits chymotrypsin-like, trypsin-like and caspase-like activities of the proteasome.

Images
(Click image to enlarge)

100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 ?M Epoxomicin for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 ?M SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader.

Equal amount of whole cell lysates prepared from DMSO (lane 1) or 20 μM Epoxomicin (lane 2)-treated HeLa cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. HEK293TCells were treated with DMSO or Epoxomicin for 4 hours.