商品编号


C3500



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E2 Conjugating Enzymes



Size

25 ?g


商品信息

Ube2R1 (UbcH3) is an E2 enzyme, which is part of the E1, E2, and E3 cascade respons
IBL
e for ubiquitination of protein substrates. Ube2R1 was first discovered during screening for cell cycle regulatory mutations that occur in yeast. While Ube2R1 is able to create diubiquitin chains by itself, it works far more efficiently and effectively with the E3 ligase Skp1 (S- phase kinase- associated protein 1)/cullin/F-box, also known as SCF, to promote substrate polyubiquitination.

Additional Information






Product Name:

6xHis-Ube2R1 (UbcH3, Cdc34)



Also Known As:

UBC3; UBCH3; UBE2R1; E2-CDC34



Catalog No.:

C3500



Size:

25 ?g



Molecular Weight:

26.7 kDa



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~90% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

NM_004359



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2R1 (UbcH3, Cdc34)







Shipping Method:

Dry ice shipping



References:

1. Plon SE,
et al
. (1993) Proc Natl Acad Sci USA 90(22), 10484 – 10488.
2. Gazdoiu S,
et al
. (2005) Proc Natl Acad Sci USA 102(42), 15053 – 15058. 3. Sadowski M,
et al
. (2007) Biochem J 405, 569 – 581.
4. Butz N,
et al
. (2005) Exp Cell Res 303(2), 482 – 493.




Details

Ube2R1 (UbcH3) is an E2 enzyme, which is part of the E1, E2, and E3 cascade respons
IBL
e for ubiquitination of protein substrates. Ube2R1 was first discovered during screening for cell cycle regulatory mutations that occur in yeast. While Ube2R1 is able to create diubiquitin chains by itself, it works far more efficiently and effectively with the E3 ligase Skp1 (S- phase kinase- associated protein 1)/cullin/F-box, also known as SCF, to promote substrate polyubiquitination.

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2R1 (UbcH3, Cdc34)