商品编号


4700-096-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


DNA Damage



Size

96 tests

商品信息

Description

This
ELISA
based assay detects poly (ADP-ribose) deposited by Tankyrase 1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a colorimetric HRP substrate yields relative absorbance that correlates with Tankyrase 1 activity.

FEATURES:


Colorimetric, non-r
ADI
oactive format

Higher throughput

96 test size

Sensitivity down to 0.1 mUnits of Tankyrase 1.


APPLICATIONS:
For the screening of candidate Tankyrase 1 inhibitors and determination of IC50 values.




Catalog # 4700-192-K includes :



Catalog Number

Description

Qty



4684-096-04

Anti-PAR monoclonal Antibody

2



4684-096-05

Goat anti-mouse IgG-HRP

2



4684-096-07

20X I-PAR Assay Buffer

2



4700-096-01

Tankyrase 1 Enzyme, 10 mUnits/?l

2



4700-096-02

Assay Substrate

2



4700-096-03

XAV939, 1 mM

2



4684-096-03

5X Antibody Diluent

2



4684-096-P

Histone-Coated Natural Strip Well Plate, I-PAR

2



4822-96-08

TACS-Sapphire?

2







Catalog # 4700-096-K includes :



Catalog Number

Description

Qty



4684-096-04

Anti-PAR monoclonal Antibody

1



4684-096-05

Goat anti-mouse IgG-HRP

1



4684-096-07

20X I-PAR Assay Buffer

1



4700-096-01

Tankyrase 1 Enzyme, 10 mUnits/?l

1



4700-096-02

Assay Substrate

1



4700-096-03

XAV939, 1 mM

1



4684-096-03

5X Antibody Diluent

1



4684-096-P

Histone-Coated Natural Strip Well Plate, I-PAR

1



4822-96-08

TACS-Sapphire?

1




“The Poly(ADP-ribose) Polymerase Enzyme Tankyrase Antagonizes
Activity of the β-Catenin Destruction Complex through
ADP-ribosylation of Axin and APC2”
“Heather E. Croy, Caitlyn N. Fuller, Jemma Giannotti, Paige Robinson, Andrew V. A. Foley, Robert J.
Yamulla, Sean Cosgriff, Bradford D. Greaves, Ryan A. von Kleeck, Hyun Hyung An, Catherine M. Powers,
Julie K. Tran, Aaron M. Tocker, Kimberly D. Jacob, Beckley K. Davis, and David M. Roberts”
J. Biol. Chem., Jun 2016; 291: 12747 – 12760.
http://www.jbc.org/cgi/content/abstract/291/24/12747