商品编号


4684-096-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


DNA Damage



Size

96 tests

商品信息

Description

This
ELISA
based assay detects poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a colorimetric HRP substrate yields relative absorbance that correlates with PARP-1 activity. ?During apoptosis PARP-1 is cleaved with a resulting drop in activity. Etoposide is included as a control apoptosis inducer.

FEATURES:


Colorimetric readout

96 well format

Highly sensitive – detects 0.1 mU PARP ~500 cells

Dynamic range between 0.1 to 10 mU PARP

Requires 10-100 ng extract for detection

Assay Time ~3 hrs


APPLICATIONS:


Measure PARP-1 activity in cell extracts before and after apoptosis.

For the screening of candidate PARP-1 inhibitors and determination of IC50 values.





Catalog # 4684-096-K includes :



Catalog Number

Description

Qty



4671-096-06

10X Activated DNA

1



4684-096-01

PARP-HSA, 10 mUnits/?l

1



4684-096-02

20 mM NAD

1



4684-096-04

Anti-PAR monoclonal Antibody

1



4684-096-05

Goat anti-mouse IgG-HRP

1



4684-096-06

10 mM Etoposide

1



4684-096-07

20X I-PAR Assay Buffer

1



4684-096-03

5X Antibody Diluent

1



4684-096-P

Histone-Coated Natural Strip Well Plate, I-PAR

1



4822-96-08

TACS-Sapphire

1




“Ultraviolet R
ADI
ation Signaling through TLR4/MyD88
Constrains DNA Repair and Plays a Role in Cutaneous
Immunosuppression”
Erin Harberts, Hua Zhou, Rita Fishelevich, Juan Liu, and Anthony A. Gaspari
J. Immunol., Apr 2015; 194: 3127 – 3135.
http://www.jimmunol.org/cgi/content/abstract/194/7/3127

“Bok Is Not Pro-Apoptotic But Suppresses Poly ADP-Ribose
Polymerase-Dependent Cell Death Pathways and Protects against
Excitotoxic and Seizure-Induced Neuronal Injury”
“Beatrice D’Orsi, Tobias Engel, Shona Pfeiffer, Saheli Nandi, Thomas Kaufmann, David C. Henshall, and
Jochen H. M. Prehn”
J. Neurosci., Apr 2016; 36: 4564 – 4578
http://www.jneurosci.org/cgi/content/abstract/36/16/4564

NAD+ repletion improves muscle function in muscular dystrophy and counters global PARylation
“Dongryeol Ryu, Hongbo Zhang, Eduardo R. Ropelle, Vincenzo Sorrentino, Davi A. G. Mázala, Laurent Mouchiroud, Philip L. Marshall, Matthew D. Campbell, Amir Safi Ali, Gary M. Knowels, Stéphanie Bellemin, Shama R. Iyer, Xu Wang, Karim Gariani, Anthony A. Sauve, Carles Cantó, Kevin E. Conley, Ludivine Walter, Richard M. Lovering, Eva R. Chin, Bernard J. Jasmin, David J. Marcinek, Keir J. Menzies, and Johan Auwerx”
Science Translational Medicine, Oct 2016; 8: 361ra139
http://stm.sciencemag.org/cgi/content/abstract/8/361/361ra139