商品编号


4685-096-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


DNA Damage



Size

96 tests

商品信息

Description

This
ELISA
based assay detects poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity. ?During apoptosis PARP-1 is cleaved with a resulting drop in activity. Etoposide is included as a control apoptosis inducer.

FEATURES:


Chemiluminescent readout

96 well format

Highly sensitive – detects 0.1 mU PARP ~500 cells

Dynamic range between 0.1 to 10 mU PARP

Requires 10-100 ng extract for detection

Assay Time ~3 hrs


APPLICATIONS:


Measure PARP-1 activity in cell extracts before and after apoptosis.

For the screening of candidate PARP-1 inhibitors and determination of IC50 values.





Catalog # 4685-096-K includes :



Catalog Number

Description

Qty



4671-096-06

10X Activated DNA

1



4684-096-01

PARP-HSA, 10 mUnits/?l

1



4684-096-02

20 mM NAD

1



4684-096-04

Anti-PAR monoclonal Antibody

1



4684-096-05

Goat anti-mouse IgG-HRP

1



4684-096-06

10 mM Etoposide

1



4684-096-07

20X I-PAR Assay Buffer

1



4675-096-01

PARP PeroxyGlow? A

1



4675-096-02

PARP PeroxyGlow? B

1



4684-096-03

5X Antibody Diluent

1



4685-096-P

Histone-Coated White Strip Well Plate, I-PAR

1