商品编号


4690-096-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


DNA Damage



Size

96 tests

商品信息

Description

4690-096-k_calculation_worksheetv2

This homogeneous assay couples PARP-1 autoribosylation to a fluorescent generating cycling assay. Inhibitors are identified by an increase in fluorescent signal when PARP-1 activity is inhibited.

FEATURES:


Highly sensitive fluorescent assay

Detect as little as 10% inhibition of PARP Activity

Inhibitors identified by increase in Fluorescent Signal

No wash steps

96 test size

Rapid – Assay takes ~ 1.5 hr


APPLICATIONS:
For the screening of candidate PARP inhibitors and determination of relative IC50 values.




Catalog # 4690-096-K includes :



Catalog Number

Description

Qty



4690-096-01

10X Cycling Enzymes

1



4690-096-08

2 ?M NAD

1



4690-096-03

PARP 1 Enzyme

1



4690-096-04

Activated DNA

1



4690-096-05

10X Resazurin

1



4690-096-06

10X Buffer

1



4690-096-07

Stop Solution

1



4690-096-P

96-well Black Microplate

1




Insights into PARP Inhibitors’ Selectivity Using Fluorescence Polarization and Surface Plasmon Resonance Binding Assays
Gianluca Papeo, Nilla Avanzi, Serena Bettoni, Antonella Leone, Mauro Paolucci, Rita Perego, Francesca Quartieri, Federico Riccardi-Sirtori, Sandrine Thieffine, Alessia Montagnoli, and Rosita Lupi
J Biomol Screen, Jun 2014; 10.1177/1087057114538319.
http://jbx.sagepub.com/cgi/content/abstract/1087057114538319v1