商品编号


C3200



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E2 Conjugating Enzymes



Size

25 ?g


商品信息

Ube2N (UbcH13/Uev1a) is a complex that acts as an E2 enzyme, which is part of the E1, E2, and E3 cascade respons
IBL
e for ubiquitination of protein substrates. Uev1a must interact with Ubc13 because it lacks the active site that other E2s posses. Ube2N has been known to catalyze K63 – linked Ub chains which aides in activation of kinases in NF – kappaB signaling pathway, amongst other tasks.

Additional Information






Product Name:

6xHis-Ube2N (6xHis-Ubc13/Uev1a)



Also Known As:

Ubc13 (UbcH-ben; MGC8489; MGC131857); Uev1a (Ube2V1; CIR1; UEV1; CROC1; UBE2V; UEV-1; CROC-1)



Catalog No.:

C3200



Size:

25 ?g



Molecular Weight:

Ubc13 (17.1 kDa), Uev1a (19.3 kDa)



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~95% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

NM_003348 (Ubc13); NM_021988 (Uev1a)



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2N (6xHis-Ubc13/Uev1a)







Shipping Method:

Dry ice shipping



References:

1. Yamaguchi T,
et al
. (1996 J Biochem 120(3), 494 – 497.
2. Hofmann RM,
et al
. (1999) Cell 96(5), 645-653. 3. Ashley C,
et al
. (2002) Gene 285(1 – 2), 183 – 191.
4. Andersen PL,
et al
. (2005) J Cell Biol 170(5), 745 – 755.




Details

Ube2N (UbcH13/Uev1a) is a complex that acts as an E2 enzyme, which is part of the E1, E2, and E3 cascade respons
IBL
e for ubiquitination of protein substrates. Uev1a must interact with Ubc13 because it lacks the active site that other E2s posses. Ube2N has been known to catalyze K63 – linked Ub chains which aides in activation of kinases in NF – kappaB signaling pathway, amongst other tasks.

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2N (6xHis-Ubc13/Uev1a)