商品编号


AG-25T-0112-R100



品牌


Adipogen



公司


Adipogen



公司分类


Antibodies



Size

100 ?l


商品信息


More Information



Product Details



Synonyms

Vasodilator-stimulated Phosphoprotein



Product Type

Polyclonal Antibody



Properties



Source/Host

Rabbit



Immunogen/Antigen

Recombinant human VASP fused to a His-tag.



Application


Immunocytochemistry
: (1:500 - 1:1000)
Western Blot
: (1:1500 - 1:3000)
Immunoprecipitation
Optimal conditions must be determined individually for each application.



Crossreactivity

Human



Specificity

Recognizes both the 46kDa (Ser
157
dephospho-) and 50kDa (Ser
157
phospho-) form of human VASP. Does not cross-react with mouse or rat VASP.



Formulation

Liquid. Crude serum containing 0.02% sodium azide.



Other Product Data


Contains Positive Control:
Human platelet protein (500?g) (Prod. No. AG-35T-0001), supplied at 5mg/ml in SDS-stop solution (100mM NaCl, 73mM Tris/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% [v/v] glycerol, 2% [w/v] SDS, 10μg/ml bromophenol blue sodium salt). Use 5μl (25μg) per lane.



Accession Number

UniProt ID P50552: VASP



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


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VASP (vasodilator-stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. VASP is an actin-associated protein involved in a range of processes dependent on
Cytoskeleton
remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping proteins. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments and it plays a role in actin-based mobility of
Listeria monocytogenes
in host cells. It regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. VASP phosphorylation is used to monitor the effect of so-called antiplatelet drugs that reduce platelet reactivity and are used to prevent stent thrombosis, strokes and heart attacks in patients. Phosphorylation of VASP at Ser157 causes a mobility shift in SDS gel electrophoresis from 46 to 50kDa, which has been used as a convenient
Marker
to monitor cyclic nucleotide-dependent protein kinase activity.