商品编号


YIF-LF-MA0319



品牌


Adipogen



公司


Adipogen



公司分类


Antibodies



Size

100 ?l

商品信息


More Information



Product Details



Synonyms

UCHL1; PGP9.5; UCH-L1; PGP 9.5; EC=3.4.19.12; Ubiquitin Thioesterase L1; Neuron Cytoplasmic Protein 9.5; Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1



Product Type

Monoclonal Antibody



Properties



Clone

AF3F8



Isotype

Mouse IgG1 κ



Immunogen/Antigen

Recombinant human GST-UCHL1 protein purified from E. coli.



Application


ELISA
Western Blot (1:500~2,000)



Crossreactivity

Human
Mouse
Rat



Purity Detail

Ammonium sulfate precipitation.



Formulation

Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.



Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.



Declaration

Manufactured by AbFrontier



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin carboxy-terminal h d l L1 hydrolase (UCHL1) is a deubiqutinating enzyme. Ubiquitin UCHL1, also known as PGP9.5, is a protein of 223 amino acids and one of the most abundant proteins in the brain (1-2% of the total soluble protein). Although it was originally characterized as a deubiquitinating enzyme recent studies indicate that it also functions as a ubiquitin (Ub) ligase and a mono-Ub st
ABI
lizer. A large amount of mono-Ub is tightly associated with UCHL1, inhibiting the degradation of mono-Ub in the brain. The precise regulation of UCHL1 is essential for neurons to survive and to maintain their proper function. UCHL1 is involved in the pathogenesis of Parkinson's disease (PD) and Alzheimer's disease (AD). Down-regulation and extensive oxidative modification of UCHL1 have been observed in the brains of AD patients as well as PD patients. A post-translational modification of UCHL1 that controls the function of UCHL1 is mono-ubiquitination. It occurs revers
IBL
y to a lysine residue near the active site of UCHL1. Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.


Product References
1) Setsuie Rand Wada K, (2007) Neu
Roche
m Int 51(2-4):105-111. (General)
2) Gong B and Leznik E, (2007) Drug News Perspect 20(6):365-370. (General)
3) Betarbet R et al., (2005) Exp Neurol 191 Suppl 1:S17-27. (General)