商品编号


AG-25T-0151-C050



品牌


Adipogen



公司


Adipogen



公司分类


Antibodies



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

UBB; Ubiquitin B



Product Type

Polyclonal Antibody



Properties



Source/Host

Rabbit



Immunogen/Antigen

Peptide sequence from ubiquitin phosphorylated at Serine 65.



Application

Western Blot (0.5-1?g/ml)



Crossreactivity

Human



Specificity

Recognizes mono- and polyubiquitin chains that are phosphorylated on Serine 65. It has no cross-reactivity with non-phosphorylated ubiquitin, ubiquitin phosphorylated at Serine 57 or Tyrosine 59, nor phosphorylated Parkin. Detects only recombinant phosporylated ubiquitin. Not recommended on natural lysates (see western blot figure).



Purity

Antigen affinity purified.



Concentration

0.5mg/ml



Formulation

Liquid. In PBS pH 7.4, 50% glycerol.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -20°C.



Documents



MSD
S


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Product Specification Sheet



Datasheet


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Serine/Threonine kinase PINK1 (PTEN-induced putative kinase protein 1) plays a critical role in preventing mitochondrial dysfunction during cellular stress. PINK1 is translated in the cytosol, then translocated to the outer mitochondrial membrane where it is rapidly cleaved and degraded as a part of normal mitochondrial function. In damaged (depolarized) mitochondria PINK1 becomes st
ABI
lized and accumulates, resulting in the subsequent phosphorylation of numerous proteins on the mitochondrial surface including Mfn2. Ultimately PARK2 (E3 Ubiquitin Ligase Parkin) is recruited to the damaged mitochondria where it is activated by PINK-mediated phosphorylation of PARK2 at serine 65, and PARK2 interaction with phosphorylated Ubiquitin (also phosphorylated by PINK on serine 65). This signaling cascade is critical for clearing the damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2.