商品编号


YIF-LF-MA0209



品牌


Adipogen



公司


Adipogen



公司分类


Antibodies



Size

100 ?l

商品信息


More Information



Product Details



Synonyms

TRBP; TARBP2; TAR RNA-binding Protein 2; RISC-lo
ADI
ng Complex Subunit TARBP2; Trans-Activation-Responsive RNA-binding Protein



Product Type

Monoclonal Antibody



Properties



Clone

46D1



Isotype

Mouse IgG1 κ



Immunogen/Antigen

Recombinant human GST-TRBP protein purified from E. coli.



Application


ELISA
Western Blot (1:5,000)



Crossreactivity

Human
Mouse
Rat



Purity Detail

Ammonium sulfate precipitation.



Formulation

Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.



Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.



Declaration

Manufactured by AbFrontier



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





TRBP (transactivation response RNA binding protein) is a protein that increases HIV-1 expression and replication by inhibition of the interferon-induced protein kinase PKR. TRBP overexpression is associated with reduced abundance of phosphorylated PKR and stimulation of protein translation. TRBP has a physiological role in spermatogenesis and growth control during development. It is oncogenic upon overexpression, likely because of its association with PKR, with the PKR activator PACT, and with the tumor suppressor Merlin. TRBP was identified as a merlin interacting protein by Gal4-based yeast two-hybrid screening of a human adult brain
CDN
A library. Merlin can inhibit tum
Origen
esis mediated by TRBP and can regulate the protein translation pathways controlled by TRBP. Dicer is a key enzyme involved in RNA interference (RNAi) and μRNA (miRNA) pathways. Ago-2, a member of the Argonaute family, Dicer and TRBP comprise the catalytic core of the RNA induced silencing complex (RISC). Required for formation of the RNA induced silencing complex (RISC). Component of the RISC lo
ADI
ng complex (RLC), also known as the μ-RNA (miRNA) lo
ADI
ng complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-α. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.


Product References
1) Christensen HS et al., (2007) J Virol. 81(10):5121-5131. (General)
2) Haase AD et al., (2005), EMBO Rep. 2005 Oct;6(10):961-7. (General)
3) Lee JY et al. (2004) . J Biol Chem. 279(29):30265-30273. (General)