商品编号


YIF-LF-MA0055



品牌


Adipogen



公司


Adipogen



公司分类


Antibodies



Size

100 ?l

商品信息


More Information



Product Details



Synonyms

TXN; ADF; Trx; TRDX; SASP; Thioredoxin; ATL-derived Factor; Surface-associated Sulphydryl Protein



Product Type

Monoclonal Antibody



Properties



Clone

3A1



Isotype

Mouse IgG2b κ



Immunogen/Antigen

Recombinant human protein purified from E. coli.



Application


ELISA
Western Blot (1:2,000)
Immunoprecipitation



Crossreactivity

Human



Purity Detail

Ammonium sulfate precipitation.



Formulation

Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.



Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.



Declaration

Manufactured by AbFrontier



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxido-reductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55 – 58 kilodalton in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various
BIOLOG
ical phenomena (1). Participates in various redox reactions through the revers
IBL
e oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the revers
IBL
e S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).


Product References
1) Mustacich, D. and Powis,G. (2000) Biochem J. 15. 346 Pt 1:1-8. (General)