商品编号


JAI-CCA-030



品牌


Adipogen



公司


Adipogen



公司分类


Assays



Size

200 tests


商品信息


More Information



Product Details



Product Type

Kit



Properties



Application Set

Quantitative
ELISA




Specificity

Specific to Ca
2+
.



Crossreactivity

All



Quantity

Enough reagents for 200 tests.



Range

0.2 - 30mg/dL



Sample Type

Cell Lysate
Plasma
Saliva
Serum
Tissue Supernatant
Urine



Detection Type

Colorimetric



Kit Contains

1 x 38 ml ? ?R-1 ? Buffer (ready to use)
1 x 10 ?ml ? R-2 ? Chelate color (ready to use)
1 x 1.0 ml ? STD ?Calcium (Ca) standard 10mg/dL (ready to use)



Other Product Data

This MC-Reagent Calcium Assay Kit is a direct colorimetric assay based on the O-Cresolphtalein (OCPC) method without deproteinization of the sample. The calcium determination is based on the reaction of calcium with O-Cresolphthalein complexone in alkaline solution, which yields a violet colored complex. The intensity of the color formed is proportional to the calcium concentration in the sample. Absorbance of the Ca
2+
-O-Cresolphtalein complex is measured at 570nm. Wavelength range of sensitivity: 560 ~ 590nm.

Features of this Assay:
Quick & Easy to use ? Species independent ? Highly sensitive, stable and suitable for high-throughput testing ? No toxic substances ? For cell lysates, serum, plasma and wide variety of
BIOLOG
ical samples



Declaration

Manufactured by JaICA.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

+4°C



Handling Advice

Do not freeze.



Use/St
ABI
lity

12 months after the day of manufacturing. See expiry date on
ELISA
Kit box.



Documents



Manual


Download PDF



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Calcium is the most abundant mineral, essential for all living organisms, where Ca
2+
sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% is present as calcium hydroxyapatite in bones and less than 1 % is present in the extra-osseous ICS (intracellular space) or ECS (extracellular space), respons
IBL
e for a myriad of important physiological effects. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Calcium ions affect the contractility of the heart and the skeletal musculature and are essential for the function of the nervous system. In addition, calcium ions play an important role in blood clotting and bone mineralization. Abnormal calcium levels have been tied to neurological, endocrine, cardiovascular, digestive, metabolic, and pulmonary diseases, as well as cancer. In plasma, calcium is bound to a considerable extent to proteins (approx. 40%), 10% is in the form of incorganic complexes and 50% is present as free (ionized) calcium. Clacium homeostasis is regulated by the parathyroid hormone (PTH), calcitriol (CT) and calcitonin. Low levels of calcium are found in hypoparathyroidism, pseudohypoparathyroidism, vitamin D deficiency, malnutrition and intestinal malabsorbtion.


Product References

Apoplastic mesophyll signals induce rapid stomatal responses to CO2 in Commelina communis: T. Fujita, et al.; New Phytol.
199,
395 (2013)

Strawberry notch homologue 2 regulates osteoclast fusion by enhancing the expression of DC-STAMP: K. Maruyama, et al.; J. Exp. Med.
210,
1947 (2013)

The pga gene cluster in Aggregatibacter actinomycetemcomitans is necessary for the development of natural competence in Ca(2+) -promoted biofilms: K. Hisano, et al.; Mol. Oral. Microbiol.
29,
79 (2014)