商品编号


4815-30-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


Apoptosis



Size

30 Samples

商品信息

Description

The labeling of tumor-containing specimens can be problematic due to the high levels of necrosis in these tissues. During necrosis, cessation of homeostasis, cell swelling, and nuclear membrane rupture generates highly degraded DNA that can interfere with interpretation of data.

Trevigen
’s kit has an optimized TUNEL-based system that preferentially labels the double-stranded DNA found in apoptotic cells. DNA fragments generated during apoptosis are end-labeled with modified nucleotides using a highly purified terminal deoxynucleotidyl transferase enzyme (TdT) in a unique non-toxic labeling buffer supplemented with a tailored cation to maximize the?signal-to-noise ratio. The incorporated nucleotides are subsequently detected using a horser
ADI
sh peroxidase conjugate. The conjugate catalyzes the conversion of diaminobenzidine (DAB) into a vis
IBL
e dark brown precipitate. The kit includes methyl green counterstain which is a general counterstain appropriate for a wide variety of tissues and tumor types and provides good contrast with the brown DAB in labeled cells. A DAB enhancer is also provided with the kit for the option to intensify or darken DAB staining.

TumorTACS? contains all the reagents required for perme
ABI
lizing, labeling, staining, and counterstaining tumor samples, including TACS-Nuclease? for preparing positive controls.

FEATURES:


Unique buffer system produces more consistent labeling.

Performance tested on tumor samples.

Includes both Proteinase K and ?exclusive non-enzymatic Cytonin? perme
ABI
lization reagent.

Includes TACS-Nuclease? solution for preparing sample-dependent positive controls.

Helps resolve unique problems encountered when using tissues or cells from tumors.


APPLICATIONS:


In situ
detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Assists in the identification of apoptotic morphologies.





Catalog # 4815-30-K includes :



Catalog Number

Description

Qty



4800-30-01

Proteinase K

1



4800-30-06

Streptavidin-HRP

1



4800-30-07

Diaminobenzidine

1



4800-30-09

DAB Enhancer

1



4800-30-15

TACS-Nuclease?

1



4800-30-16

TACS-Nuclease? Buffer

1



4800-30-18

Methyl Green 1%

1



4810-30-02

TACS? 2 TdT Labeling Buffer

1



4810-30-03

TACS? 2 TdT Stop Buffer

1



4810-30-04

TACS? 2 TdT dNTP

1



4810-30-05

TdT Enzyme

1



4810-30-14

50x Manganese Cation

1



4876-05-01

Cytonin?

1




“Anticancer drug candidate CBL0137, which inhibits histone chaperone FACT, is efficacious in preclinical orthotopic models of temozolomideresponsive and -resistant glioblastoma”
“Tara A. Barone, Catherine A. Burkhart, Alfiya Safina, Gary Haderski, Katerina V. Gurova, Andrei A.Purmal, Andrei V. Gudkov, and Robert J. Plunkett”
Neuro Oncology, Jul 2016; 10.1093/neuonc/now141.
http://neuro-oncology.oxfordjournals.org/cgi/content/abstract/now141v1