商品编号


4823-30-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


Apoptosis



Size

30 Samples

商品信息

Description

The kit has been developed to overcome the common difficulties unique to neuronal samples including the fragile nature of brain tissue sections, high background problems, poor counterstaining with common dyes, and the need to perform dual labeling experiments to detect cell specific antigens in conjunction with apoptotic cells. A key feature is NeuroPore?, a proprietary perme
ABI
lization reagent that gently perme
ABI
lizes samples while retaining cell morphology. NeuroPore? also contains blocking reagents to allow its use as an antibody diluent in immunohistochemistry and to reduce background staining.

DNA fragments generated by apoptosis are end-labeled with modified nucleotides using a highly purified terminal deoxynucleotidyl transferase enzyme (TdT). The incorporated nucleotides are detected using a horser
ADI
sh peroxidase system that catalyzes the conversion of diaminobenzidine (DAB) into a vis
IBL
e dark brown precipitate. NeuroTACS? II contains the Blue Counterstain to allow visualization of all cells within the sample with good contrast to the brown DAB precipitate. A DAB enhancer is also provided with the kit for the option to intensify or darken DAB staining. In addition, the Blue Counterstain is compat
IBL
e with the red-colored substrates used for phosphatase detection in double labeling experiments. The protocol includes details for labeling
in situ
for apoptosis and antigen detection on the same sample. To ensure that your own samples have been processed and perme
ABI
lized correctly, we provide TACS-Nuclease?, a unique reagent used to generate a positive control with your own samples. This provides a high degree of confidence for data interpretation and can help pinpoint problem steps in the labeling procedure.

FEATURES:


Unique buffer system produces more consistent labeling.

Performance tested on brain sections.

Includes exclusive NeuroPore? perme
ABI
lization reagent.

Includes TACS-Nuclease? solution for preparing sample-dependent positive controls.

Helps resolve unique problems encountered when detecting apoptotic neuronal cells.


?

APPLICATIONS:


In situ
detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Assists in the identification of apoptotic morphologies.





Catalog # 4823-30-K includes :



Catalog Number

Description

Qty



4800-30-01

Proteinase K

1



4800-30-06

Streptavidin-HRP

1



4800-30-07

Diaminobenzidine

1



4800-30-09

DAB Enhancer

1



4800-30-15

TACS-Nuclease?

1



4800-30-16

TACS-Nuclease? Buffer

1



4810-30-02

TACS? 2 TdT Labeling Buffer

1



4810-30-03

TACS? 2 TdT Stop Buffer

1



4810-30-04

TACS? 2 TdT dNTP

1



4810-30-05

TdT Enzyme

1



4810-30-14

50x Manganese Cation

1



4820-30-01

NeuroPore?

1



4820-30-13

Blue Counterstain

1




?

Differences in the Phagocytic Response of Microglia and Peripheral Macrophages after Spinal Cord Injury and Its Effects on Cell Death,
Andrew D. Greenhalgh and Samuel David
J. Neurosci., Apr 2014; 34: 6316 – 6322.
http://www.jneurosci.org/cgi/content/abstract/34/18/6316

Hypoxia Induc
IBL
e Factor-1{alpha} (HIF-1{alpha}) Is Required for Neural Stem Cell Maintenance and Vascular St
ABI
lity in the Adult Mouse SVZ
Lu Li, Kate M. Candelario, Kelsey Thomas, Ruth Wang, Kandis Wright, Amber Messier, and Lee Anna Cunningham

Synaptic Synthesis, Dephosphorylation, and Degradation: A NOVEL PAR
ADI
GM FOR AN ACTIVITY-DEPENDENT NEURONAL CONTROL OF CDKL5
Paolo La Montanara, Laura Rusconi, Albina Locarno, Lia Forti, Isabella Barbiero, Marco Tramarin, Chetan Chandola, Charlotte Kilstrup-Nielsen, and Nicoletta Landsberger
J. Biol. Chem., Feb 2015; 290: 4512 – 4527.
http://www.jbc.org/cgi/content/abstract/290/7/4512