商品编号


3471-096-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


Cell Based Assays



Size

96 tests

商品信息

Description

The Cultrex
?

In Vitro
Angiogenesis Assay Endothelial Cell Invasion Kit was created in an effort to accelerate the screening process for compounds that influence vascular endothelial cell digestion of and invasion through a basement membrane extract (BME) layer. This kit offers a flex
IBL
e, standardized, 96 well high-throughput format for quantifying endothelial invasion,
in vitro
, in response to chemoattractants and/or inhibiting compounds. This assay employs a simplified Boyden chamber-like design with an 8 micron polyethylene terephthalate (PET) membrane. Sulforaphane [1-isothiocyanato-(4R)-methylsulfinyl)-butane], a naturally occurring cancer chemopreventive agent, is provided as a control for inhibition of
in vitro
endothelial cell invasion through Cultrex
?
BME. Detection of cell invasion is quantified using Calcein-AM, which?is internalized by the cells, and converted to the acetomethylester (AM) moiety by intracellular esterases. Free Calcein fluoresces brightly, and this fluorescence may be used to quantify the number of cells that have invaded using a fluorescence plate reader.




Catalog # 3471-096-K includes :



Catalog Number

Description

Qty



3455-096-02

5X BME Coating Solution

1



3470-096-02

Sulforaphane

1



4892-010-01

Calcein AM

1



3455-096-01

Cell Invasion Chamber

1



3455-096-03

10X Coating Buffer

1



3455-096-04

25X Cell Wash Buffer

1



3455-096-05

10X Cell Dissociation Solution

1