商品编号


GKE-5016D



品牌


ProZyme



公司


ProZyme, Inc



公司分类


Glyko Enzymes



Size

1 U

商品信息

Description

PNGase F, peptide-
N
-glycosidase F,
peptide-
N
4
-(
N-
acetyl-β-glucosaminyl)asparagine amidase.
?Releases intact N-glycans by cleaving
?between the innermost GlcNAc and Asn.?Same manufacturing process as N-Glycanase for GlykoPrep (EDTA-free), supplied at the?
GKE-5006
?concentration of?≥ 2.5 U/ml.

Source:
Recombinant gene from
Elizabethkingia
meningosepticum
, expressed in
E. coli
. The source organism was previously known as?
Chryseobacterium [Flavobacterium] meningosepticum
[1].
?

Specificity:?
Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide-bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free.





?





Figure 1:

Cleavage specificity for GKE-5016 N-Glycanase (PNGase F).
R
1
: N & C substitution other than H
R
2
:?may be H or the rest of an oligosaccharide
R
3
:?may be H or?
α(1-6)-linked fucose

"A & B" pack sizes ship with:



1 ea WS0010 5x N-Glycanase Reaction Buffer

1 ea WS0012 Denaturation Solution

1 ea WS0013 Detergent Solution

1 ea WS0145 5x N-Glycanase Tris Reaction Buffer (MS applications)



"D" pack size does not ship with the above buffers.?Buffers are available with "D" pack size
on request
.

Formulation:

20 mM Tris-HCl, 50 mM NaCl (pH 7.5)
Reaction Buffer:

5X concentrated buffer, when diluted gives 20 mM sodium phosphate pH 7.5, containing 0.02% sodium azide.
MW:

~35,000 Daltons
pH optimum/range
:
8.6 / 7.5 - 9.5
Unit Definition:

One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5.

Not formulated for FACE? analysis, use GK50050 instead.

Size:
1 U in 400 ?l

Concentration:
?≥ 2.5 U/ml

Product Code:
GKE-5016D

1. Kim KK, Kim MK, Lim JH, Park HY, Lee ST.?Transfer of
Chryseobacterium meningosepticum
and
Chryseobacterium miricola
to
Elizabethkingia
gen. nov. as
Elizabethkingia meningoseptica
comb. nov. and
Elizabethkingia miricola
comb. nov.?
Int J Syst Evol Microbiol.
2005 May;55(Pt 3):1287-93.