商品编号


M4050



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Ubiquitin / UBL Derivatives



Size

1 mg

商品信息

Class II fluorescence polarization assay reagent for deubiquitylating enzymes, based on the peptide sequence 26–39 of ubiquitin. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys33 to Ub via a native isopeptide bond. See references 4-6 for full assay and experimental details.


Additional Information






Product Name:

TAMRA-Ub26-39(K33)-Ub



Also Known As:

N/A



Catalog No.:

M4050



Size:

1 mg



Molecular Weight:

10.5 kDa



Species:

Human



Source:

Synthetic



Stock:

Lyophilized powder



Concentration:

N/A



Quality Assurance:

≥95% by RP-HPLC and SDS-PAGE (CBB and fluorescence sacnning). Protein content verified by SDS-PAGE.



Storage:

Store powder and DMSO stock at ?20°C, aqueous stocks at ?80°C. Protect from light. Please avoid multiple freeze/thaw cycles.



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

N/A



Image(s):

N/A



Shipping Method:

Wet ice shipping



References:

1. Tirat et al. Anal. Biochem. 2005, 343, 244.
2. Huang et al. Methods in Molecular
BIOLOG
y 2009, 565, 127.
3. Levine et al. Anal. Biochem. 1997, 247, 83.
4. Faesen et al. Chem. Biol. 2011, 18, 1550.
5. Geurink et al. ChemBiochem 2012, 13, 293.
6. Mevissen et al. Cell 2013, 154, 169.





Details

Class II fluorescence polarization assay reagent for deubiquitylating enzymes, based on the peptide sequence 26–39 of ubiquitin. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys33 to Ub via a native isopeptide bond. See references 4-6 for full assay and experimental details.