商品编号


AG-20T-0208-C050



品牌


Adipogen



公司


Adipogen



公司分类


Antibodies



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

UBB; Ubiquitin B



Product Type

Monoclonal Antibody



Properties



Clone

83406



Isotype

Mouse IgG2b



Immunogen/Antigen

Synthetic human ubiquitin+1 peptide.



Application


Western Blot (0.1-0.5?g/ml)




Crossreactivity

Human



Specificity


Recognizes human Ubiquitin and Ubiquitin+1.




Purity Detail

Protein A or G purified.



Formulation

Liquid. In PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast ubiquitin, and 100% aa sequence identity shared between human and mouse ubiquitin. In mammals, four ubiquitin genes encode for two ubiquitin-ribosomal fusion proteins and two poly-ubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of
BIOLOG
ical processes such as signal transduction, endocytosis, and post-endocytic sorting.