商品编号


AG-27B-0009-C100



品牌


Adipogen



公司


Adipogen



公司分类


Antibodies



Size

100 ?g


商品信息


More Information



Product Details



Product Type

Recombinant Antibody



Properties



Clone

MB11



Isotype

Human IgG2&lam
BD
a;



Source/Host

Purified from?HEK 293 cell?culture supernatant.



Immunogen/Antigen

Full length GTP-&lam
BD
a;-S loaded tubulin from pig brain.



Application


Immunocytochemistry:
(1:10'000)



Crossreactivity

Drosophila
Human
Mouse
Rat



Specificity

Recognizes human, mouse, rat and drosophila tubulin-GTP.



Purity

≥95% (SDS-PAGE)



Purity Detail

Protein A-affinity purified.



Concentration

1mg/ml



Formulation

Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.



Other Product Data

anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is composed of human variable regions (VH and VL) (&lam
BD
a;-chain) of immunoglobulin fused to the human lgG2 Fc domain.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 month after receipt when stored at +4°C.
Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.


Product References

Detection of GTP-Tubulin Conformation in Vivo Reveals a Role for GTP Remnants in Microtubule Rescues: A. Dimitrov, et al.; Science
322,
1353 (2008)

Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport: T. Nakata, et al.; J. Cell Biol.
194,
245 (2011)